Title: Research Associate III, Antibody Discovery & Engineering
Location: Bothell, WA
Duration: 8 months
Job Description:
- Supports a broad variety of research studies to assess the value of health care interventions.
- Works with project teams to manage the day-to-day activities of studies.
- Collaborates with Medical and Marketing staff to ensure consistent Outcomes Research messages.
- Database analysis, chart reviews, patient surveys, literature reviews, decision models, budget impact models, systematic reviews, meta-analyses, product strategic planning, and cost-effectiveness analyses.
- Assists in the process of abstracts/posters, PowerPoint presentations and publications.
- Manages relationships/coordinates activities such as advisory panels and best practice sharing with users of the program.
- Participate in development of antibody-based oncology therapeutics.
- The Scientist will report to the Molecular and Cellular Technologies team lead within Antibody Discovery.
- Their primary responsibilities will include engineering cell lines and performing flow-based assays to support antibody campaigns.
- The successful candidate will work well in a highly collaborative team environment and possess expertise in flow cytometry, mammalian cell culture, and cell engineering.
Responsibilities:
- Design, execute, troubleshoot, optimize, and interpret experiments.
- Order constructs for overexpression and knockout cell line generation.
- Engineer cell lines using transfection, nucleofection, CRISPR/Cas9, lentiviral transduction, and other methodologies.
- Evaluate target expression on engineered and endogenous cell lines using flow cytometry and molecular characterization.
- Perform routine cell culture maintenance and banking, working with both primary and cancer cells.
- Optimize and perform flow cytometry assays to screen and characterize antibodies.
- Maintain laboratory spaces, equipment, and supplies.
- Keep an up-to-date and accurate electronic laboratory notebook.
- Present experimental findings and contribute to scientific discussion at group and cross-functional team meetings.
Required Qualifications:
- Bachelor’s degree + 4 years laboratory research experience or master's degree + 2 years laboratory research experience in biological sciences or related subject.
- Proficiency in culturing/maintaining multiple cell lines under BSL2 conditions.
- Knowledge of cell engineering platforms, including transient transfection, generation of stable cell lines, and CRISPR.
- Experience in multi-color flow cytometry.
Preferred Qualifications:
- Experience with FACS and single cell cloning.
- Basic molecular biology skills, including vector design, cloning, and PCR.
- Knowledge of antibodies or antibody drug conjugates (ADCs).