Neurodegenerative diseases are one of the largest medical challenges of our time. Denali Therapeutics is a biotechnology company dedicated to developing breakthrough therapies for neurodegenerative diseases through our deep commitment to degeneration biology and principles of translational medicine. Denali is founded on the collaboration of leading scientists, industry experts, and investors who share the vision that scientific discovery energetically applied to translational medicine is the key to delivering effective therapies to patients.
We invite you to consider an opportunity with Denali to help achieve our goal of delivering meaningful therapeutics to patients.
Responsibilities
- Develop and troubleshoot protein expression and purification processes for large molecule biotherapeutics.
- Lead the production of large molecule biotherapeutics from milligram to gram scale, including development of robust and scalable purification processes and fit-for-purpose analytics that support the identification and characterization of novel therapeutics.
- Evaluate and establish technologies to advance generation of bispecific antibodies (e.g. high-throughput production and characterization approaches).
- Develop and perform protein purification, biophysical and biochemical characterizations, supporting and meeting timelines for production of a wide variety of proteins, including antibodies, enzymes, Fc-fusions, antigens (soluble / secreted but also membrane proteins targets) and protein complexes.
- Analyze proteins using standard methods (including HPLC, SEC-MALS, DLS, DSF)
- Coordinate activities and work collaboratively with colleagues across Discovery and Development to meet timelines and to help advance programs.
- Actively contribute to project teams and work effectively across functional teams to help advance programs to development.
Requirements
- PhD in Biochemistry, Biophysics, Structural Biology or a or related field; or a BS/MS with 6+ years of experience.
- Extensive knowledge and hands-on experience in the purification and analytical characterization of challenging proteins, complexes and fusion proteins.
- Extensive programming experience of protein purification platforms (FPLC/HPLC/robotics).
- Demonstrated and documented experience in producing and assessing the purity and homogeneity of complex proteins such as engineered antibodies (e.g. bispecifics, Fc-fusions and/or bioconjugated therapeutics) and generation of challenging proteins to support immunization campaigns.
- Experience in all aspects of protein purification, including chromatographic separation by gravity and liquid chromatography methods, all relevant analytics for the quality assessment of purified protein (which may include intact mass spectrometry, ligand binding assessment by SPR or related methods), and possibly some structural biology experience (sample preparation for crystallography and electron microscopy studies and/or basic knowledge of structural determination workflows).
- SPR / BLI experience is a plus.
- Excellent oral, presentation and written communication skills are required.
- Ability to work in a highly dynamic, fast paced environment to contribute to multiple projects.
- Self-motivated with excellent record keeping and written and verbal communication skills.
- Creative thinker with a strong sense of responsibility and a desire to collaborate in an exciting and fast-paced research environment
Salary Range: $123,000.00 to $153,000.00 . Compensation for the role will depend on a number of factors, including a candidate’s qualifications, skills, competencies, and experience. Denali offers a competitive total rewards package, which includes a 401k, healthcare coverage, ESPP and a broad range of other benefits. Learn more at https://www.denalitherapeutics.com/careers
This compensation and benefits information is based on Denali’s good faith estimate as of the date of publication and may be modified in the future.This compensation and benefits information is based on Denali’s good faith estimate as of the date of publication and may be modified in the future.
Denali is committed to its core company value of unity by creating a diverse and inclusive environment. We are proud to be an equal opportunity employer and do not discriminate against any employee or applicant for employment because of race, color, sex, age, national origin, religion, sexual orientation, gender identity and/or expression, status as a veteran, basis of disability, or any other federal, state, or local protected class.